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KMID : 0385019860020010009
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Korean Journal of Laboratory Animal Science
1986 Volume.2 No. 1 p.9 ~ p.14
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Phytomitogen Induced Blast Transformation of Rabbit:Ⅰ. Effect of Medium, Phytomitogen and Culture Hours on the Uptake of 3H-Thymidine
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Abstract
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The present study has been carried out to investigate the optimal condition on lymphocyte blastogenesis of rabbit lymphocytes, whole blood culture and microculture system in conjunction with a semiautomatic multiple sample harvester(SAMSH) was used to study the in vitro optimal condition of rabbit lymphocytes.
Data were presented to show many variables that are involved in studying the phytohemagglutmin(PHA) and lipopolysaccharide reponse of rabbit lymphocyte in a microculture system. Analysis indicated that the conditions for optimal PHA as measured by incorporation of H-TdR include:
1. Use of RPMI-1640 as culture medium.
2. Use of 6 ㎍ of PHA, per culture.
3. 48-hour culture period.
Conditions for optimal stimulation with LPS nitogen were similar to those used for PHA.
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KEYWORD
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